ISOLATION OF SOIL FUNGI AND FERMENTATION CONDITIONS OF FUSARIUM SP. AGAINST STAPHYLOCOCCUS AUREUS
Abstract
- The present study, soil samples were collected from six different places at Wundwin Township in Mandalay Region. The isolation of fungi were isolated by using physical treatment dilution method (Hayakawa and Kobayashi, 2005) and direct method (Ando,2004).Then, antimicrobial activities of soil fungi were tested by paper disc diffusion assay method. The fungal extract were tested for antimicrobial activity against test organisms Staphylococcus aureus. In the fifteen fungi screening, the isolated fungi TW-03 (22.57 mm) and TW-14(34.81mm)exhibited the activity against Staphylococcus aureus. In this result, TW-14 showed highest antibacterial activity. Fusarium sp. was identified from the selected fungus TW-14. Therefore, this fungus Fusarium sp. was selected for further investigation such as age, size, carbon sources and nitrogen sources for suitable fermentation condition. In the study of ages and sizes of inoculum, it was observed that 60hr and 10% seed cultured was best for the fermentation. Then, in this study of carbon and nitrogen sources utilization it was found that soluble starch and soybean gave the best activity on Staphylococcus aureus.
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Year
- 2020
Author
-
Thet Thet Wai
Subject
- Botany
Publisher
- Myanmar Academy of Arts and Science (MAAS)