FERMENTATION STUDIES OF CEPHALOSPORIUM SP. ISOLATED FROM HESPERETHUSA CRENULATA (ROXB.) ROEM.
Abstract
- Endophytic fungal strain Cephalosporium sp. was isolated from the wood of Hesperethusa crenulata (Roxb.) Roem. For the fermentation conditions such as various carbon and nitrogen sources, culture media, age of inoculum, size of inloculum and pH utilization of this strain Cephalosporium sp. were conducted at Microbiology Lab, Department of Botany, University of Yangon. In utilization of carbon sources, honey and sucrose were good whereas yeast extract, meat extract and malt extract were the best nitrogen sources. In antimicrobial activity of various carbon sources, sucrose medium showed very high activity whereas various nitrogen sources, yeast extract medium indicated very high activity against eight test organisms. In the investigation of the morphological characters on various media, the seven cultural media were good media. According to the result of antimicrobial activity on various media, sucrose/yeast extract medium was the best for fermentation. In the study of inoculum optimization, two days old (age of inoculum) and 1.5% of seed culture at fifth day fermentation were suitable for the production of bioactive metabolites from this strain. In the study of pH utilization, pH 7 was the best for extraction of the bioactive compounds.
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Year
- 2020
Author
-
Htet Htet Zaw
Subject
- Botany
Publisher
- Myanmar Academy of Arts and Science (MAAS)