ISOLATION AND ANTIMICROBIAL ACTIVITY OF ENDOPHYTIC CTINOMYCETES FROM DIFFERENT PARTS OF FIVE SELECTED PLANTS AND CHARACTERIZATION OF SELECTED STREPTOMYCES (TG-16)
Abstract
- A total of eighteen endophytic actinomycetes were isolated from leaf, stem, root of five selected plants such as Millingtonia hortensis L., Aloe vera L., Barleria strigosa Willd., Desmodium triquetrum (L.)DC. and Polygonum chinensis L. from Pathein University Campus from June to August, 2016. This study was carried out at Biotechnological Development and Resources Centre, Pathein University. The isolated strains were designated as TG 1-18. All of the isolated strains were studied their morphological, microscopical characters and Gram staining. The antimicrobial activity of eighteen isolated strains were studied by agar well diffusion method with ten kinds of test organisms. Among them, ten strains showed the antimicrobial activity on Agrobacterium tumefaciens, Aspergillus paraciticus, Salmonella typhi, Escherichia coli and Saccharomyces cerevisae. TG-16 showed the highest antimicrobial activity (27.14mm) against E. coli. Therefore, TG-16 was selected and characterized by biochemical reactions. The selected strain TG-16 was gram positive with sporophore as hook, open-loop rectinaculum apertum type, spiral spore chain and spore smooth and oval shape. The colony morphology of TG-16 was raised, entire and circular. In the sugar fermentation test of selected strain TG-16, the acid was produced on all studied carbon sources except galatose, lactose, arabinose and positive results to methyl red, nitrate reduction, citrate utilization, catalase, arginine and casein hydrolysis, growth on streak line of potato slice and sodium chloride level at 2.5%(w/v), melanin was not produced but yellow pigment produced in casein hydrolysis medium. According to the results, TG-16 was characterized as the genus Streptomyces.
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Year
- 2020
Author
-
Theingi Aung
Subject
- Botany
Publisher
- Myanmar Academy of Arts and Science (MAAS)