ISOLATION AND ANTIMICROBIAL ACTIVITY OF BACTERIA FROM MANGROVE SOIL AND BIOCHEMICAL CHARACTERIZATION OF SELECTED BACTERIUM (ZM-7)
Abstract
- Mangrove soil samples were collected from four different station of U-To Creek at Shwe thaung yan Township (Ma-Gyi), Ayeyawady Region. These samples were cultured on Nutrient Agar (NA) and American Type Culture Collection (ATCC) medium. A total of 26 bacterial colonies were obtained and 14 strains from Nutrient Agar (NA) and 12 isolates from (ATCC) medium. Isolated strains were designated as ZM 1 to 26. These isolated strains were tested by using ten different test organisms from one day to three days old culture. Five strains showed the antimicrobial activity. Among them, ZM-7 showed the highest antimicrobial activity (24.34 mm) on Escherichia coli and (22.63 mm) on Candida albicans. Therefore, ZM-7 was selected and characterized by morphological, microscopial, Gram staining and biochemical characteristics. In the colony morphology, ZM-7 was medium in size, entire, creamy, flat and creamy glistening. In the microscopical and biochemical characteristics, ZM-7 was Gram positive and short rod, catalase positive, aerobes and acid was produced in the sugar fermentation and gas did not produce. According to the results, ZM-7 was characterized as the possible genus Bacillus.
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Year
- 2020
Author
-
Zin Mar Cho
Subject
- Botany
Publisher
- Myanmar Academy of Arts and Science (MAAS)