PHENOLIC CONTENT, ANTIOXIDANT AND ANTITUMOUR ACTIVITIES AND ISOLATION OF A PHENOLIC COMPOUND FROM THE BARK OF ALSTONIA SCHOLARIS L. (TAUNG-MA-YO)
Abstract
- The present work focused on the determination of total phenol content, antioxidant activity and screening of anti-tumour activity of some crude extracts of the bark of Alstonia scholaris L. (Taung-ma-yo). Moreover, the research deals with the isolation of a phenolic compound by column chromatography and identification of the isolated compound by UV and FT IR. The total phenol contents of crude extracts of 70 % ethanol (99.33 g GAE/mg) and water (61.5 g GAE/mg) were determined by spectrometric Folin-Ciocalteu reagent (FCR) method .The antioxidant activity of 70 % ethanol extract (IC50 value of 27.75 g/mL) and water extract (IC50 value of 90.56 g/mL) of the bark sample was determined by DPPH assay method. Preliminary screening of anti-tumour activity of crude extracts of ethyl acetate, 70 % ethanol and water was carried out by Potato Crown Gall test. It was observed that, of the three doses, i.e., 0.05, 0.1 and 0.15 g/disc each extract tested, the minimum dose showing activity was 0.15 g for ethyl acetate extract and 0.05 g for 70 % ethanol extract on day 5. On day 7, tumours appeared for the ethyl acetate extract but not for the 70 % ethanol extract as observed under microscope and by staining with iodine solution. Column chromatographic separation of ethyl acetate extract by gradient elution with pet ether, ethyl acetate and methanol yielded a compound (A1) having Rf value of 0.45 on silica gel layer with pet ether : ethyl acetate (2.5 : 7.5, v/v). UV spectra of isolated compound (A1) showed that it was to be a phenolic compound by alkaline red shift and also supported by FT IR spectrum.
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Year
- 2020
Author
-
Khin Maw Maw
Subject
- Chemistry
Publisher
- Myanmar Academy of Arts and Science (MAAS)