FERMENTATION STUDIES OF IOACTIVE FUNGAL STRAIN MM4 ISOLATED FROM THE LEAVES OF SM AJAA L.
Abstract
- In this study, bioactive strain MM4 isolated from the leaves of Psidium guaavaL. was utilized to investigate optimal fermentation conditions such as carbon and nitrogen sources, various culture media, age of inoculum, size of inoculum and pH utilization. In carbon sources, sucrose, starch and glycerol were the best whereas yeast, meat, malt and soybean were good nitrogen sources. In antimicrobial activity, sucrose, glucose, starch and mannitol in carbon sources showed high activity against Candida albicans, Escherichia coli and Malasseia furfur while in nitrogen sources, yeast extract, meat extract, and soybean indicated very high activity against Candida albicansand Escherichia coli. In the investigation of morphological characters on eleven media, fermentation media 9, 7 and 10 were good to produce antimicrobial metabolites from strain MM4. Antimicrobial activity of eleven media, fermentation medium 9 showed highest activity against test organisms. Two days old age of inoculum showed highest activity against test organisms. According to the results, size of inoculum (1.5%) and pH 4 with two days old age of inoculum were the best for extraction of the bioactive compounds from fermented broth.
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Year
- 2018
Author
-
Hnin Wit Mhon.
Subject
- Botany
Publisher
- Myanmar Academy of Arts and Science (MAAS)