STUDY ON ANTIOXIDANT ACTIVITY, ANTIMICROBIAL ACTIVITY AND ACUTE TOXICITY OF PLUKENETIA VOLUBILIS L. (SACHA INCHI) LEAVES
Abstract
- This research focused on the investigation of phytochemical constituent, mineral content, antioxidant, antimicrobial activities and acute toxicity of sacha inchi leaves. Firstly, the preliminary phytochemical screening was done. The leaves give positive test for alkaloid, flavonoid, glycoside, terpene, steroid, reducing sugar, saponin, tannin and phenolic compound but cyanogenic glycoside was not found. The mineral content was determined by EDXRF spectroscopic technique. The main minerals present in sacha inchi leaves are calcium and potassium. The antioxidant activity of ethanol extract was determined by DPPH assay. The IC50 value of ethanol extract was found to be 179.60 µgmL-1 . Moreover, the antimicrobial activity of ethanol extract was investigated by agar well diffusion method on seven selected microorganisms. Among selected microorganisms, the ethanol extract showed the highest activity on Bacillus pumilus and high activities on Agrobacterium tumefaciens, Bacillus subtilis, Candida albicans, Escherichia coli, Pseudomonas fluorescens and Staphylococcus aureus. In addition, oral acute toxicity test of ethanol extract was studied by Organization of Economic Cooperation and Development (OECD) guideline (425). According to oral acute toxicity test, the tested sacha inchi leaves can be considered relatively safe to the dose level of 5000 mg/kg body weight. No toxicity effects after oral acute exposure of ethanol extract to mice were observed.
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Year
- 2021
Author
-
Myint Myint Htay
Subject
- Chemistry
Publisher
- Myanmar Academy of Arts and Science (MAAS)