INVESTIGATION OF PHYTOCONSTITUENTS AND SOME BIOACTIVITIES OF LEAVES AND BARKS OF HOLOPTELEA INTEGRIFOLIA R. (PHYAUK-SEIK)
Abstract
- Due to the increasing resistance of pathogens to the antibiotics, the plant kingdom is more focused than ever because the most parts of the plants have the ability to synthesize a wide variety of chemical compounds that possess important biological functions. The selected Myanmar medicinal plant, Holoptelea integrifolia R. (Phyauk-seik), belonging to family Ulmaceae, is known to have many bioactivities. This research deals with phytochemical and medico-chemical investigations of leaves and barks of H. integrifolia. The preliminary phytochemical investigation revealed the presence of many valuable phytochemicals in both samples. Based on DPPH assay method, the 70% EtOH extracts of leaves and barks of H. integrifolia showed the mild antioxidant activity by comparing with the standard ascorbic acid. The antimicrobial activity of PE, EtOAc and 70 % EtOH extracts of leaves and barks were screened on six microorganisms, namely B. substilis, S. aureus, P. fluorescens, A. flavus, C. albicans and E. coli by paper disc diffusion assay. All of the tested extracts, except PE extract of barks exhibited the antimicrobial activity with inhibition zone diameters ranged between 8 mm ~ 18 mm for leaves and 10 mm ~ 22 mm for barks, while the PE extract of barks did not show the activity. The antiproliferative activity of PE, EtOAc and 70 % EtOH extracts of leaves and barks of H. integrifolia were determined by MTT assay on two human cancer cell lines: A 549 (lung) and HeLa (cervix). All of the tested extracts of leaves exhibited mild activities on two tested cancer cell lines with IC50 values > 100 µg/mL. And, PE and 70 % EtOH extracts of barks exhibited significant activities on two tested cell lines with IC50 values < 20 µg/mL, except the EtOAc extract, while the EtOAc extract of barks showed mild activity on cervix cancer cell line with IC50 value > 100 µg/mL.
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Year
- 2021
Author
-
Tha Zin Nwe Oo
Subject
- Chemistry
Publisher
- Myanmar Academy of Arts and Science (MAAS)