EFFECT OF CARBON AND NITROGEN SOURCES ON ANTIBACTERIAL METABOLITE PRODUCTION BY ENDOPHYTIC FUNGUS ASPERGILLUS CANDIDUS AGAINST AGROBACTERIUM TUMEFACIENS
Abstract
- In present study, endophytic fungus was isolated from the petiole of Vigna mungo (L.) Hepper in Thae Phyu Village, Hinthada Township, Ayeyawady Region during June in 2018. The endophytic fungus was isolated by surface sterilization method. The effect of various carbon and nitrogen sources were observed for the colony characters, growth condition and maximum antibacterial metabolite production of isolated fungus. The isolated fungus was identified as Aspergillus candidus based on morphological-microscopical characters and references key. The antibacterial activities of Aspergillus candidus were studied by agar well diffusion assay method. The result showed that maximum production of antibacterial metabolite (20.69 mm) was obtained when medium was supplemented with rice powder as carbon source. Fermentation medium containing yeast extract as nitrogen source showed the highest antibacterial metabolite (22.80 mm) production. This study reveals that endophytic fungus serves as a potential source for the production of an effective compound.
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Year
- 2021
Author
-
Khin Thida Swe
Subject
- Botany
Publisher
- Myanmar Academy of Arts and Science (MAAS)