OPTIMIZATION OF FERMENTATION CONDITIONS, EXTRACTION OF CRUDE EXTRACT AND IDENTIFICATION OF SOIL FUNGUS MM-34
Abstract
- In the present study, twenty different soil samples were collected from twenty different places of Belu-Gyun, Chaung-Zon Township, Mon State. Totally 37 fungi were isolated from 20 soil samples. Among these 37 isolates, MM-34 was found to show maximum antibacterial activity, in compare to other isolates. Thus, soil fungus MM-34 was selected for the study of effects of pH on fermentation. Initial pH of 5.0 was the best for fermentation conditions. Maximum antibacterial activity (31.01 mm inhibitory zone) against Micrococcus luteus was observed in pH 5.0. Different temperature such as 20°C, 25°C, 30°C, 35°C and 40°C was studied for maximum production of antibacterial activity by soil fungus MM-34. Maximum antibacterial activity by fungal isolate MM34 was recorded at temperature 30°C (30.52 mm inhibitory zone) against Micrococcus luteus. In the study of static and shaking culture, static culture was optimal condition for MM-34 fermentation (30.68 mm inhibitory zone). Based on the all optimum fermentation conditions, the selected soil fungus MM-34 was carried out by fermentation period. It was observed that the culture which were incubated for 6 days shows maximum antibacterial activity (34.84 mm inhibitory zone) against Micrococcus luteus. According to the Rf values, ethyl acetate is suitable for the extraction of crude extract from the fermented broth. Crude extract of ethyl acetate was observed antibacterial activity (26.19 mm inhibitory zone) against Micrococcus luteus. The TLC plates were developed in the solvent of chloroform and chloroform-methanol mixture (9:1, 8:2, 7:3, 6:4, 4:6 and 3:7) and Hexane only and Hexane-ethyl acetate mixture (9:1, 8:2, 7:3, 6:4, 5:5, 4:6 and 3:7). Based on the morphological and microscopical characters, soil fungus MM-34 was identified as Penicillium sp.
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Year
- 2021
Author
-
Mya Min Min Myo
Subject
- Botany
Publisher
- Myanmar Academy of Arts and Science (MAAS)