GENE EXPRESSION OF DLNPGR1 IN DIFFERENT TISSUES, DURING SE AND SEED GERMINATION IN DIMOCARPUS LONGAN LOUR.
Abstract
- Two important structural units, tetratricopeptide repeat (TPR) motifs and calmodulin-binding domain, are known for their involvement in protein-protein interaction and binding to calmodulin (CaM), respectively. Although many TPR-containing proteins, CaM-binding proteins (CBPs) and their respective encoding genes have been studied, further detail studies are still needed for more understanding of those important proteins and their respective genes. In this study, expression of a gene encoding CBP with TPRs, NPGR1, was analysed in different tissues, during SE and germination in Dimocarpus longan Lour. By using RT- PCR analysis, the expression levels of DlNPGR1 in 12 different tissues, in different developmental stages during longan somatic embryogenesis (SE), and during seed germination in different days after sowing. According to our results, among 12 different tissues, the relatively highest expression level was detected in young fruit, followed by floral bud and female flower while mature root, pollen and mature leave exhibited the lowest expression. During SE, the highest expression of DlNPGR1 was detected in NEC followed by CE while the lowest expression level was detected in EC stage. During seed germination, the highest expression of was detected in day 0 then the expression was significantly lower in day 4 followed by day 8 where the expression level was the lowest. In conclusion, being a gene encoding CBP with TPRs, DlNPGR1 might be involved in cell division.
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Year
- 2021
Author
-
Min Kyaw Thu
Subject
- Botany
Publisher
- Myanmar Academy of Arts and Science (MAAS)