ISOLATION OF A BIOACTIVE SESQUITERPENOID COMPOUND AND EVALUATION OF SOME BIOLOGICAL ACTIVITIES OF STEMS AND ROOTS OF ALLAMANDA CATHARTICA L. (SHWEWA-PAN)
Abstract
- This research focused on the screening of phytochemical constituents, nutritional values and biological activities such as antioxidant, antimicrobial and anti-tumor activities of the stems and roots of Allamanda cathartica L. (Shwewa-pan). These samples were collected from Bago University Campus and identified at Botany Department, Bago University. According to the phytochemical tests, alkaloids, flavonoids, terpenoids, steroids, glycosides, organic acids, phenolic compounds, saponins, tannins and carbohydrates were found to be present in both samples. The nutritional values were determined by AOAC method resulting 6.91 and 18.64 % of proteins, 2.04 and 3.04 % of moisture, 2.71 and 3.06 % of ash, 62.42 and 38.65 % of fiber, 10.33 and 16.15 % of fat in stems and roots of A. cathartica, respectively. By thin layer and silica gel column chromatographic methods, one compound, plumericin (1.51 %, m.pt 210°C) was isolated from ethyl acetate extract of the roots. The antioxidant activity of ethanol and watery extracts of the stems and roots determined by DPPH radical scavenging activity assay was found to be in the order of stem ethanol extract (IC50= 65.89 μg/mL) > root ethanol extract (IC50= 110.46μg/ mL) > root watery extract (IC50>200 μg/mL) ≈ stem watery extract (IC50> 200 μg/mL) . The antimicrobial activity of PE, EtOAc, EtOH and H2O extracts of stems and roots were screened on six microorganisms such as Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus, Candida albicans and Escherichia coli by agar well diffusion method. All of the extracts from the roots have more pronounced antimicrobial activity with inhibition zone diameters ranged between 14 mm ~ 35 mm than stems extracts (12 mm ~ 18 mm). Among them EtOAc extract of the roots has the highest activity against B. pumilus. Antitumor activity of various crude extracts such as PE, EtOAc and EtOH extracts of stems and roots in different concentrations (0.5, 1.0, 1.5g/mL) and plumericin (10, 20, 30 mg/mL) were also determined on tumor producing bacteria by using PCG (Potato Crown Gall) test. It was found that EtOAc extract of roots, PE and EtOH extracts of both samples and plumericin exhibited the inhibition of tumor formation.
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Year
- 2019
Author
-
Yin Yin Tun
Subject
- Chemistry
Publisher
- Myanmar Academy of Arts and Science (MAAS)