ANTIOXIDANT ACTIVITY, CHOLESTEROL LOWERING EFFECT AND CHEMICAL INVESTIGATION OF PO-SA (STEM)
Abstract
- Po-sa (Morus alba L.) plant possesses many useful biological activities which has been the reason for the selection of this plant for the present study. The present work is concerned with the detection of antioxidant and cholesterol lowering activities of Po-sa (stem). Antioxidant activity of Po-sa (stem) extracts was determined by using 1,1-Diphenyl- 2-picrylhydrazyl assay method. The IC50 values of EtOAc, EtOH and watery extracts of stem were 2.94μg/mL, 1.21μg/mL and 1.20 μg/mL, respectively. The EtOH and watery extracts of stem showed more radical scavenging activity than EtOAc extract. Cholesterol lowering activity of crude extracts of stem was also studied by Zlatkis, Zak and Boyle method on rats model. The cholesterol levels were determined after orally administrated rats with 600 mg/kg/day of plant extracts for 10 days. It indicated that watery extract of stem has the ability to reduce bad cholesterol (Triglyceride, Total cholesterol and Very low density lipoprotein) and it also could effectively increase the good blood cholesterol (High density lipoprotein) level. By using silica gel column chromatographic separation method, umbelliferone (0.001 %) and scopoletin (0.002 %) were obtained from the EtOAc extract of the stem. The isolated compounds were identified and elucidated by melting points and by using joint application of modern spectroscopic techniques such as UV-visible, FT IR, 1 H NMR and mass spectrometry, compared with the reported data. Umbelliferone and scopoletin isolated from Po-sa(stem) also showed radical scavenging activity determined by using semi-quantitative DPPH staining method. The evaluation of in-vitro antioxidant activity of isolated compounds showed up to 6.25 μg/mL.
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Year
- 2019
Author
-
Ei Ei Sann
Subject
- Chemistry
Publisher
- Myanmar Academy of Arts and Science (MAAS)