FERMENTATION, EXTRACTION AND IDENTIFICATION OF SELECTED SOIL FUNGUS (YTF 8) FROM KAN GYI DAUNG TOWNSHIP, AYEYARWADY REGION
Abstract
- Soil samples were collected from 5-different places of Kan Gyi Daung Township. Eighteen fungi were isolated from these 5-different soil samples. Soil was analyzed at the Applied Geology Department, Yangon University. Isolation of fungi was undertaken by the physical and chemical treatment method. The soil fungi were isolated by using Low Carbon Agar medium for first culture and Potato Glucose Agar medium for pure culture. The isolated fungi were given as temporary names YTF 1 to YTF 18. The purpose of this study was to screen for antimicrobial metabolite production and effect of variation of carbon and nitrogen sources. In this study, thirteen fungal strains were tested by agar well diffusion method with three kinds of test organisms. YTF 1, 2, 7, 8, 9, 11, 12, 15, 16, 17 and 18 showed antimicrobial activity and the remaining five fungi YTF 3, 4, 10, 13 and 14 did not show the activity. The selected fungi (YTF 1, 7, 8, 11, 15 and 16) showed antimicrobial activity against on Agrobacterium tumefaciens, while YTF 1, 2, 7, 8, 15 and 16 showed the activity on Candida albicans. Especially YTF 8 gave the best activities on Candida albicans. The fermentation conditions of YTF 8 was carried out by the studies of proper age, size and different carbon and nitrogen sources on antimicrobial metabolite production on Candida albicans. The 5 days was found to be optimum fermentation period. In the size of inoculum, 5 %, 15 % and 20 % were used and 20 % was suitable condition. Moderate growth and the best antimicrobial production of selected fungus (YTF 8) was observed in both carbon and nitrogen sources. The addition of potato as a carbon source resulted in the maximum growth and the inhibition zones reached 33.96 mm in YTF 8. YTF 8 showed the moderate growth on almost all nitrogen sources. The inhibition zone of YTF 8 was 32.90 mm on peptone. The present study was focused on fermentation, extraction and identification of selected soil fungus. Firstly, fermentation was carried by the fermentation medium (FM) with the different concentrations of carbon and nitrogen sources. In the different carbon concentrations, FM 6 (glucose 1.5 g) gave the moderate antifungal activity (39.62 mm) followed by using (glucose 1 g) in FM 5 (31.52 mm) and (glucose 2 g) in FM 7 (31.51 mm) against Candida albicans respectively. In the nitrogen concentrations, FM 14 (peptone 1 g) exhibited the highest activity (30.83 mm). And then, the effect of pH was studied by varying pH of 4, 5, 6, 7, 8, 9 and 10. In extraction of antifungal metabolites, YTF 8 was observed by four solvents. Among the four solvents, ethyl acetate was found as the most suitable solvent and Rf value had 0.81. And then, the fungal culture filtrates were studied by various ratios (1:1, 2:1, 3:1 v/v) of ethyl acetate and various pH. Among them, the ethyl acetate extract (1:1) demonstrated the highest activity (30.45 mm) at pH 6 showed highest inhibitory effect against Candida albicans. Finally, the selected fungus YTF 8 was identified. According to the results, the surface color of YTF 8 was pale yellow and the reverse colour was yellow. The microscopic character of YTF 8 was conidial heads on PGA radiate, conidiophores: long, vesicle: pyriform, sterigmata: uniseriate, phialides: ampliform. Therefore, YTF 8 was identified as Aspergillus clavatus.
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Year
- 2019
Author
-
Yee Yee Thin
Subject
- Chemistry
Publisher
- Myanmar Academy of Arts and Science (MAAS)