INVESTIGATION ON SOME PHYTOCHEMICAL CONSTITUENTS AND BIOACTIVITIES OF MADHUCA LONGIFOLIA L. (MYINTZU-THAKA-NATPAN) LEAVES
Abstract
- Some phytochemical constituents from the leaves of Madhuca longifolia L. (Myintzu-tha-ka-natpan) and its biological activities such as antioxidant and antimicrobial activities were investigated. Preliminary phytochemical tests have revealed that the presence of alkaloids, flavonoids, α-amino acids, organic acids, phenolic compounds, saponins, glycosides, cyanogenic glycosides, tannins, steroids and terpenoids in the sample. The leaves of sample was relatively high Ca, K and S according to EDXRF spectrum. In vitro antioxidant activities of 95 % EtOH and watery extracts from M. longifolia leaves were assessed by DPPH radical scavenging activity assay. IC50 values were found to be 6.64 μg/mL for H2O extract and 3.62 μg/mL for 95 % EtOH extract of M. longifolia leaf. The in vitro antimicrobial activities of PE, EtOAc, CHCl3, 95 % EtOH and H2O extracts from M. longifolia leaves were screened by agar well diffusion method on six species of microorganisms, namely Bacillus pumilus, Bacillus subtilis, Candida albicans, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The inhibition zone diameters were found in the range between 14-19 mm for ethanol extract, 15-17 mm except Pseudomonas aeruginosa organisms for PE extract, 12-16 mm except Escherichia coli for chloroform extrat , 13-15 mm for ethyl acetate extract and 11-27mm except Bacillus pumilus organisms for water extract. Therefore , ethyl acetate extract exhibited the highest antimicrobial activities that followed by EtOH extract, PE extract, water extract and CHCL3 extract. Therefore, on the basis of the results that have been observed in the present study, it can be inferred that the leaves may have therapeutic potential as remedy by virtue of its biochemical activities.
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Year
- 2019
Author
-
Myin Myint Khaing
Subject
- Chemistry
Publisher
- Myanmar Academy of Arts and Science (MAAS)