Abstract

• In this research, Leea macrophylla Roxb. ex Hornem., a medicinal plant native to Myanmar, was selected for investigation of its phytochemical constituents, antibacterial activity, antioxidant property, anti-inflammatory effect. It gave rise to positive tests for alkaloids, flavonoids, glycosides, phenols, polyphenols, saponins, steroids, reducing sugars, terpenes and tannins, respectively. The antimicrobial activity of crude extracts from this sample was tested by using paper disc diffusion assay method on six selected microorganisms. The results indicated that the ethyl acetate extract exhibited the highest antimicrobial activity against all tested organisms compared to the other extracts. Moreover, the antioxidant activity of ethyl acetate extract and n hexane extract of this sample was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay method using UV-Visible spectrophotometer. The ethyl acetate extract revealed stronger antioxidant activity with an IC50 value of 20.341 μg/mL compared to the IC50value of 31.313 g/mL for the n-hexane extract. In addition, the anti-inflammatory activity of the ethanol extract was assessed by Croton Oil-Induced Ear Edema method. The results showed that the anti inflammatory activity of groups A, B and C showed (36 % for 0.75 mg/ear, 45 % for 1.15 mg/ear, and 48 % for 2.25 mg/ear) respectively.