Abstract

• There are various DNA extraction kits available on the market for animal tissue. In the present study, three DNA extraction kits, utilizing the silica solution-based extraction method, the GS solution-based method, and the Proteinase K solution-based method, were employed to extract total genomic DNA from Clarias batrachus, and their extraction efficiencies were compared. The quantity and quality of the extracted DNA were evaluated using a Nanodrop spectrometer. The Silica solution-based method yielded DNA concentrations of 19.1 ng/µl, 20.8 ng/µl, and 25.9 ng/µl, with A260/280 purity ratios of 2.05, 2.06, and 1.99, and A260/A230 contamination ratios of 0.06, 0.05, and 0.06, respectively, for three specimens of catfish. The GS solution-based method produced DNA concentrations of 167.2 ng/µl, 75.6 ng/µl, and 372.0 ng/µl, with purity ratios of 1.92, 2.09, and 1.99, and contamination ratios of 0.80, 0.85, and 1.30. The proteinase K solution based method resulted in DNA concentrations of 124.2 ng/µl, 164.0 ng/µl, and 85.6 ng/µl, with purity ratios of 2.14, 2.09, and 2.14, and contamination ratios of 1.67, 1.73, and 1.35. Time analysis showed that the Proteinase K solution-based method was the fastest, requiring 22 minutes, while the Silica solution-based method and the GS solution-based method took 93 minutes and 82 minutes, respectively. This study highlights the differences in extraction efficiency, quality, and time consumption among the three methods.