PREPARATION AND CHARACTERIZATION OF HYDROXYAPATITE-MGO NANOCOMPOSITES AND THEIR ANTIMICROBIAL ACTIVITY AND ACTE TOXICITY
Abstract
- The application of pure hydroxyapatite (HAp) are restricted to non load-bearing implants due to the poor mechanical properties of hydroxyapatite. To improve the mechanical properties of hydroxyapatite derived from cow bone, incorporation of magnesium oxide was conducted in this research. Hydroxyapatite was prepared by calcination of deproteinised cow bone waste using HCl and NaOH solutions. Magnesium oxide was prepared by wet chemical method treating magnesium nitrate solution with sodium hydroxide solution followed by thermal decomposition of magnesium hydroxide at 600 C. After addition of magnesium oxide to HAp, the XRD pattern showed the two new peaks corresponding to magnesium oxide peaks at (200) and (220). Crystallite sizes of hydroxyapatite - MgO nanocomposites were 32.17 nm and 37.87 nm for HAp-5 % MgO nanocomposites calcined at 1000 C and 1100 C respectively. For HAp-10 % MgO nanocomposites the crystallite sizes were 31.46 nm and 36.70 nm, respectively for calcination temperature of 1000 C and 1100 C. Crystal structures of all HAp-MgO nanocomposites and HAp were indexed as hexagonal. FT IR spectral data revealed the characteristics peaks of both hydroxyapatite and MgO in the prepared nanocomposites. HAp-MgO nanocomposites showed mild antimicrobial activities on all tested organisms such as Bacillus subtilis, Pseudomonas aeruginosa, Candida albicans, Escherichia coli, Staphylococcus aureus and Bacillus pumilus. In vivo acute toxicity test on albino mice showed no mortality and no toxicity throughout the dosing schedule of 14 days at all dose levels in all groups.
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Year
- 2018
Author
-
Cho Lwin Lwin Khine
Subject
- Chemistry.IC
Publisher
- Myanmar Academy of Arts and Science (MAAS)