INVESTIGATION OF SOME BIOACTIVITIES OF Peperomia pellucida L. (THIT-A-GI) AND Enhydra fluctuans L. (KANA-PHAW)
Abstract
- In the present work, Peperomia pellucida L. (Thit–yay–gyi) and Enhydra fluctuans L. (Kana-phaw) were chosen to investigate some bioactivities such as antimicrobial, antioxidant, antitumor, anticancer or antiproliferative activities. The antimicrobial activity of different crude extracts from the Thit-yay-gyi and Kana-phaw was determined by agar well diffusion method. Ethyl acetate extracts of both plants samples showed the antimicrobial activity on all tested microorganisms with inhibition zone diameters in the range between 18 mm-35 mm for Thit-yay-gyi and 21 mm35 mm for Kana-phaw. The antioxidant activity of ethanol and watery crude extracts of Thit-yay-gyi and Kana-phaw were investigated by using DPPH free radical scavenging assay. The antioxidant activity of Kana-phaw ethanol extract (IC50 = 22.53 µg/mL) was found to be highest followed by Thit-yaygyi ethanol extract (IC50 = 37.75 µg/mL), then by Kana-phaw watery extract (IC50 = 40.63 µg/mL) and Thit-yay-gyi watery extract (IC50 = 44.81 µg/mL). In addition, the cytotoxicity of EtOH and H2O crude extracts from Thit-yay-gyi and Kana-phaw were evaluated by brine shrimp cytotoxicity bioassay. The cytotoxicity of Thit-yay-gyi EtOH extract (LD50=68.2µg/mL) showed more cytotoxic effect than other crude extracts. The antitumor activity of crude extracts was pre-screened by PCG test. It was found that they can inhibit the growth of tumor in the rangs of concentration 1 g/mL, 0.5 g/mL and 0.25 g/mL, 0.12 g/mL. Furthermore, antiproliferative activity of EtOH and H2O extracts of Kana-phaw and Thityay-gyi was investigated by using seven human cancer cell lines. Antiproliferative activity of two extracts were found to be in order of EtOH extract of Thit-yay-gyi>EtOH extract of Kana-phaw> H2O extract of Thityay-gyi> H2O extract of Kana-phaw. Among the tested crude extracts, EtOH extract of Thit-yay-gyi were found to be more potent than other crude extracts. Therefore, it could be inferred that EtOH extract of Thit-yay-gyi possessed the higher antiproliferative activity than Kana-phaw. By silica gel column chromatographic separation technique, compound A (kaurenoic acid) (white crystal, 0.015 %, m.pt. 172 C) from PE extract of Kana-phaw was isolated. The isolated compound was identified by physicochemical properties and modem spectroscopic technique such as FT IR, NMR, HMBC and ESI MS spectrometry as well as by comparing with their reported data. Isolated compound A (kaurenoic acid) was evaluated by brine shrimp cytotoxicity bioassay. Compound A (kaurenoic acid) showed 50 % death of brike shrimp at concentration of 0.00001 mg/mL.
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Year
- 2018
Author
-
Mai Thet Mar Tun
Subject
- Chemistry.IC
Publisher
- Myanmar Academy of Arts and Science (MAAS)